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Type of publication: Journal Article
Type of document: Full Paper

Year: 2018

Authors: Kotob, MH; Kumar, G; Saleh, M; Gorgoglione, B; Abdelzaher, M; El-Matbouli, M

Title: Differential modulation of host immune genes in the kidney and cranium of the rainbow trout (Oncorhynchus mykiss) in response to Tetracapsuloides bryosalmonae and Myxobolus cerebralis co-infections.

Source: Parasit Vectors. 2018; 11(1):326



Authors Vetmeduni Vienna:

El-Matbouli Mansour
Gorgoglione Bartolomeo
Kotob Mohamed Hosny Abdelhafez
Kumar Gokhlesh
Saleh Mona

Vetmed Research Units
University Clinic for Poultry and Fish Medicine, Clinical Unit of Fish Medicine


Project(s): The role of SOCS Proteins in salmonid whirling disease


Abstract:
Most of the studies on fish diseases focus on single infections, although in nature co-infections occur more often. The two freshwater myxozoan parasites of salmonids, having high economic and ecologic relevance are Tetracapsuloides bryosalmonae (Malacosporea), the etiological agent of proliferative kidney disease, and Myxobolus cerebralis (Myxosporea), the etiological agent of whirling disease. The present study aims to investigate immune modulation in rainbow trouts (Oncorhynchus mykiss) during single and co-infections by these parasites.Fish were initially infected with T. bryosalmonae (one group) and M. cerebralis (another group) separately. At 30 days post-exposure (dpe), both the single species infected groups were co-infected, respectively, with the other parasite. Posterior kidney and cartilage cranium samples were collected at 30, 60, 90 and 120 dpe and RT-qPCR was performed on them to assess the transcription of suppressors of cytokine signaling (SOCS) -1 and -3, Janus kinase-1 (JAK-1) and signal transducer and activator of transcription-3 (STAT-3) genes.Kidney samples from the T. bryosalmonae-infected group showed upregulation of all immune genes tested between 60-120 dpe. Crania from the single M. cerebralis-infected group and the M. cerebralis and T. bryosalmonae co-infected group exhibited upregulation of SOCS-1 and JAK-1 between 60-120 dpe and SOCS-3 at 120 dpe. However, only in the single M. cerebralis-infected group, was a statistically significant expression of STAT-3 observed at 30 and 60 dpe.The results of this study indicate that both T. bryosalmonae and M. cerebralis induce overexpression of SOCS-1 and SOCS-3 genes and modulate the host immune response during the development of parasite to cause immunosuppression.

Keywords Pubmed: Animals
Coinfectionveterinary
Fish Diseasesimmunologyparasitology
Immunosuppressionveterinary
Janus Kinasesgenetics
Kidneyparasitology
Myxobolusimmunology
Myxozoaimmunology
Oncorhynchus mykissgeneticsimmunologyparasitology
Parasitic Diseases, Animalimmunologyparasitology
Real-Time Polymerase Chain Reactionveterinary
STAT Transcription Factorsgenetics
Signal Transduction
Skullparasitology
Suppressor of Cytokine Signaling Proteinsgenetics

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