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Publication type: Journal Article
Document type: Full Paper

Year: 2004

Author(s): Hoegen, B; Saalmüller, A; Röttgen, M; Rziha, HJ; Geldermann, H; Reiner, G; Pfaff, E; Büttner, M

Title: Interferon-gamma response of PBMC indicates productive pseudorabies virus (PRV) infection in swine.

Source: Vet Immunol Immunopathol. 2004; 102(4):389-397

Authors Vetmeduni Vienna:

Saalmüller Armin

Vetmed Research Units
Institute of Immunology

In Chinese Meishan/German Landrace cross-bred swine F2 generation interferon gamma (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) was determined directly ex vivo at different time points after survival of a virulent pseudorabies virus (PRV) infection. This reactivity was compared with the reactivity of naïve PBMC. Significant IFN-gamma production was determined in ELISA and ELISPOT only after in vitro PBMC re-stimulation with PRV and not with the closely related bovine herpesvirus BHV-1. The PRV-specific IFN-gamma secretion from re-stimulated PBMC showed high levels 6 days after infection, before the presence of serum antibodies, and it persisted at a high level over a 3 months period. The response of a group of eight piglets infected intranasally with PRV varied. Only two animals showed the expected typical fever response. PRV specific IFN-gamma production by PBMC clearly indicated that infection had occurred. Early significant IFN-gamma production by primed PBMC turned out to be a reliable and specific ex vivo marker for cellular response against productive PRV infection in swine before antibody formation.

Keywords Pubmed: Animals
Antibodies, Viral/blood
Antigens, Viral/immunology
Enzyme-Linked Immunosorbent Assay/veterinary
Herpesvirus 1, Suid/immunology
Leukocytes, Mononuclear/immunology*
Swine Diseases/immunology*
Swine Diseases/virology

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