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Publication type: Journal Article
Document type: Full Paper

Year: 2005

Author(s): Wienhold, D; Armengol, E; Marquardt, A; Marquardt, C; Voigt, H; Büttner, M; Saalmüller, A; Pfaff, E

Title: Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination.

Source: Vet Res. 2005; 36(4):571-587



Authors Vetmeduni Vienna:

Saalmüller Armin

Vetmed Research Units
Institute of Immunology


Abstract:
The aim of this study was to determine the immunomodulatory effects of IL-12, IL-18 and CD154 (CD40 ligand, CD40L) in DNA-vaccination against the classical swine fever virus. Four recombinant plasmids were constructed including the CSFV coding region for the glycoprotein gp55/E2 alone or together with porcine IL-12, IL-18 or CD154 genes. Five groups of four pigs each were immunized intramuscularly (i.m.) three times with the respective constructs. The control group was inoculated with empty plasmid DNA. Eighteen days after the final immunization, the pigs were challenged with a lethal dose of CSFV strain Eystrup and monitored for a further 16 days. This study showed that co-delivery of IL-18 and CD154 induced an earlier appearance of serum antibodies, reduced B-cell deficiency after infection and protected pigs against a lethal CSFV infection. In contrast, co-delivery of IL-12 led to a reduced titer of neutralizing antibodies and protection against a lethal CSFV challenge in comparison to the other pigs and to pigs that were immunized with a gp55/E2 plasmid alone.

Keywords Pubmed: Adjuvants, Immunologic/genetics*
Animals
CD40 Ligand/genetics
CD40 Ligand/immunology
Classical Swine Fever/prevention & control*
Classical swine fever virus/immunology*
Cytokines/genetics*
Cytokines/immunology
Gene Expression
Glycoproteins/immunology
Interleukin-12/genetics
Interleukin-12/immunology
Interleukin-18/genetics
Interleukin-18/immunology
Plasmids
Swine
Time Factors
Vaccines, DNA/immunology*
Viral Proteins/immunology
Viral Vaccines/immunology*
Viremia


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