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Type of publication: Journal Article
Type of document: Full Paper

Year: 2019

Authors: Loncaric, I; Kübber-Heiss, A; Posautz, A; Ruppitsch, W; Lepuschitz, S; Schauer, B; Feßler, AT; Krametter-Frötscher, R; Harrison, EM; Holmes, MA; Künzel, F; Szostak, MP; Hauschild, T; Desvars-Larrive, A; Misic, D; Rosengarten, R; Walzer, C; Slickers, P; Monecke, S; Ehricht, R; Schwarz, S; Spergser, J

Title: Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals.

Source: Vet Microbiol. 2019; 230:138-144

Authors Vetmeduni Vienna:

Desvars Amelie
Krametter-Frötscher Reinhild
Kübber-Heiss Anna
Künzel Frank
Loncaric Igor
Posautz Annika
Rosengarten Renate
Spergser Joachim
Szostak Michael
Walzer Christian

Vetmed Research Units
University Clinic for Small Animals, Clinical Unit of Internal Medicine Small Animals
University Clinic for Ruminants, Clinical Unit of Ruminant Medicine
Research Institute of Wildlife Ecology, Conservation Medicine
Institute of Microbiology
Research Institute of Wildlife Ecology

The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecR1-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.Copyright © 2019 Elsevier B.V. All rights reserved.

Keywords Pubmed: Animals
Animals, Domesticmicrobiology
Animals, Wildmicrobiology
Anti-Bacterial Agentspharmacology
Bacterial Proteinsgenetics
DNA, Bacterialgenetics
Drug Resistance, Multiple, Bacterial
Methicillin Resistancegenetics
Microbial Sensitivity Tests
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Staphylococcusdrug effectsgeneticsisolation & purification

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