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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2019

Authors: Papp, SM; Fröhlich, T; Radefeld, K; Havlicek, V; Kösters, M; Yu, H; Mayrhofer, C; Brem, G; Arnold, GJ; Besenfelder, U

Title: A novel approach to study the bovine oviductal fluid proteome using transvaginal endoscopy.

Source: Theriogenology. 2019; 132:53-61

Authors Vetmeduni Vienna:

Besenfelder Urban
Brem Gottfried
Havlicek Vitezslav
Itze-Mayrhofer Corina
Papp Sophie
Radefeld Karina

Vetmed Research Units
Institute of Animal Breeding and Genetics, Unit of Reproductive Biology

Project(s): Optimisation of early reproductive success in dairy cattle through the definition of new traits and improved reproductive biotechnology

The oviduct provides the optimal micro milieu for early embryo development. However, accessing the bovine oviductal fluid in vivo for analysis is still challenging and therefore the oviductal fluid is usually collected post mortem. In the study presented here we introduce a novel approach to gain minimal invasive access to the bovine oviductal fluid proteome in vivo by transvaginal endoscopy at different stages of the estrous cycle. The first experiment aimed at transferring C4 derivatised magnetic beads to bind the oviductal fluid proteome in situ. Protein carrying beads were recovered by flushing the oviduct and proteins were eluted. In the second experiment a flushing solution was injected into and aspirated from the oviduct repeatedly. The flushing solution was centrifuged to separate the fluid from the cellular debris. Proteins were identified by nano-LC-MS/MS. Two different stages of the estrous cycle (Day 1 and Day 3) were analyzed in samples from 30 heifers. Both methods were applied successfully and in total, more than 3000 proteins were identified, so far representing the most comprehensive OF proteome published. This new minimal invasive approach to access the bovine oviductal fluid proteome facilitates future innovative experimental designs to study the role of the oviductal micro environment during early embryo development.Copyright © 2019 Elsevier Inc. All rights reserved.

Keywords Pubmed: Animals
Body Fluidschemistry
Chromatography, Liquid
Estrous Cyclemetabolism
Fallopian Tubesphysiology
Gene Expression Regulation
Tandem Mass Spectrometry

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