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Type of publication: Journal Article
Type of document: Full Paper

Year: 2019

Authors: Rushton, JG; Korb, M; Kummer, S; Reichart, U; Fuchs-Baumgartinger, A; Tichy, A; Nell, B

Title: Protein expression of KIT, BRAF, GNA11, GNAQ and RASSF1 in feline diffuse iris melanomas.

Source: Vet J. 2019; 249:33-40



Authors Vetmeduni Vienna:

Fuchs-Baumgartinger Andrea
Kummer Stefan
Nell Barbara
Reichart Ursula
Rushton Jessica
Stargardt Melanie
Tichy Alexander

Vetmed Research Units
University Clinic for Small Animals, Clinical Unit of Small Animal Surgery
Institute of Pathology
Platform Bioinformatics and Biostatistics
VetCore


Abstract:
Feline iris melanoma, the most common feline intraocular tumour, has a reported metastatic rate of 19-63%. However, there is a lack of knowledge about its molecular biology. Previous studies have reported that feline iris melanomas do not harbour mutations comparable to common mutations found in their human counterpart. Nevertheless, there are differences in the gene expression patterns. The aim of this study was to investigate the protein expression of B-RAF oncogene serine/threonine kinase (BRAF), G protein subunit alpha q (GNAQ) and 11 (GNA11), KIT proto-oncogene receptor tyrosine kinase (KIT), and Ras association family member 1 (RASSF1) in feline iris melanomas. Fifty-seven formalin-fixed paraffin embedded (FFPE) iris melanomas and 25 FFPE eyes without ocular abnormalities were stained with antibodies against the respective proteins using immunofluorescence. Averaged pixel intensities/μm2 and percentage of stained area from total tissue area were measured and the results were compared. Compared to the control group, iris melanomas showed overexpression of BRAF, GNAQ, GNA11 and KIT. The higher expression of BRAF, GNAQ, GNA11 and KIT in feline iris melanomas suggest that these proteins may play a key role in the development of feline iris melanomas and KIT may present a possible target for future therapies in cats with feline iris melanomas.Copyright © 2019 Elsevier Ltd. All rights reserved.

Keywords Pubmed: Animals
Cat Diseasesmetabolism
Cats
Female
Fluorescent Antibody Techniqueveterinary
GTP-Binding Protein alpha Subunitsbiosynthesis
GTP-Binding Protein alpha Subunits, Gq-G11biosynthesis
Iris Neoplasmsmetabolismveterinary
Melanomametabolismveterinary
Protein Biosynthesis
Proto-Oncogene Proteins B-rafbiosynthesis
Proto-Oncogene Proteins c-kitbiosynthesis
Tumor Suppressor Proteinsbiosynthesis

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