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Selected Publication:

Type of publication: Journal Article
Type of document: Note

Year: 2019

Authors: Zwirzitz, B; Wetzels, SU; Rabanser, I; Thalguter, S; Dzieciol, M; Wagner, M; Mann, E

Title: Culture-Independent Evaluation of Bacterial Contamination Patterns on Pig Carcasses at a Commercial Slaughter Facility.

Source: J Food Prot. 2019; 82(10):1677-1682



Authors Vetmeduni Vienna:

Dzieciol Monika
Selberherr Evelyne
Wagner Martin
Wetzels Stefanie
Zwirzitz Benjamin

Vetmed Research Units
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Microbiology


Project(s): Austrian Competence Centre for Feed and Food Quality, Safety and Innovation


Abstract:
Traditionally, the microbiological status of meat is determined by culture-based techniques, although many bacteria are not able to grow on conventional media. The aim of this study was to obtain quantitative data on total bacterial cell equivalents, as well as taxa-specific abundances, on carcass surfaces during pig slaughter using quantitative real-time PCR. We evaluated microbial contamination patterns of total bacteria, Campylobacter, Escherichia coli, Lactobacillus group, Listeria monocytogenes, Salmonella, and Pseudomonas species throughout slaughtering and on different carcass areas. In addition, we compared contamination levels of breeding sow carcasses with fattening pig carcasses, and we assessed the efficacy of carcass polishing machines under two water amount conditions. Our results demonstrate that relevant meat-spoilage organisms show similar contamination patterns to total bacteria. The highest bacterial load was detected in the stunning chute (4.08 × 10


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