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Type of publication: Journal Article
Type of document: Full Paper

Year: 2005

Authors: Wieser, M; Worliczek, H; Kämpfer, P; Busse, HJ

Title: Bacillus herbersteinensis sp. nov.

Source: Int J Syst Evol Microbiol. 2005; 55(Pt 5):2119-2123



Authors Vetmeduni Vienna:

Busse Hans-Jürgen
Wieser Monika
Worliczek Hanna

Vetmed Research Units
Institute of Microbiology


Project(s): Experimental decay of wall paintings by microorganisms ¿ proof of principle and strategies of prevention


Abstract:
Two bacterial strains, designated D-1,5a(T) and D-1,5b, were isolated from a medieval wall painting in the chapel of Castle Herberstein, Styria (Austria). The Gram-positive, heterotrophic, aerobic, spore-forming rods showed nearly identical whole-cell protein patterns, identical genomic fingerprints and identical physiological profiles, demonstrating their relationship at the species level. Both strains contained meso-diaminopimelic acid in their peptidoglycan, possessed a quinone system comprising menaquinone MK-7 and had fatty acid profiles in which C(15:0) iso and C(15:0) anteiso were predominant. The 16S rRNA gene sequence of D-1,5a(T) showed the highest similarity (99.5%) to the sequence of Bacillus sp. LMG 20243, and Bacillus flexus IFO 15715(T) was the next most closely related established species (96.5%). Other type strains, such as Bacillus fastidiosus DSM 91(T), Bacillus indicus SD/3(T), Bacillus cibi JG-30(T), Bacillus megaterium IAM 13418(T), Bacillus cohnii DSM 6308(T), Bacillus bataviensis LMG 21833(T) and Bacillus soli LMG 21838(T), shared 96.0-96.1% 16S rRNA gene sequence similarity with D-1,5a(T). The combination of physiological and chemotaxonomic traits distinguishes the two strains from those species sharing the highest sequence similarities (96.0-96.5%). On the basis of these characteristics and the phylogenetic position of strain D-1,5a(T) (=DSM 16534(T)=CCM 7228(T)), this strain is assigned as the type strain of a novel species of the genus Bacillus, for which the name Bacillus herbersteinensis sp. nov. is proposed.

Keywords Pubmed: Austria
Bacillus/chemistry
Bacillus/classification*
Bacillus/genetics
Bacillus/isolation & purification
Bacterial Typing Techniques
DNA, Bacterial/analysis
DNA, Ribosomal/analysis
Fatty Acids/analysis
Genes, rRNA
Humans
Molecular Sequence Data
Paintings*
Phenotype
Phylogeny
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Species Specificity


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