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Type of publication: Journal Article
Type of document: Full Paper

Year: 2020

Authors: Graage, R; Beck, S; Koch, M; Dolezal, M; Schwarz, L; Hennig-Pauka, I

Title: Vergleich von Speichelproben und ­gepoolten Serumproben für den ­Nachweis von Antikörpern gegen das Porzine Reproduktive und Respiratorische ­Syndrom Virus in ­Aufzuchtbeständen.

Other title: Comparison between oral fluid samples and pooled serum samples for the detection of antibodies against Porcine Reproductive and Respiratory Syndrome Virus in weaning pig herds

Source: Schweiz Arch Tierheilkd. 2020; 162(9):531-538



Authors Vetmeduni Vienna:

Dolezal Marlies
Hennig-Pauka Isabel
Schwarz Lukas

Vetmed Research Units
Platform Bioinformatics and Biostatistics
University Clinic for Swine


Abstract:
Monitoring of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in pig farms is performed usually by testing for antibodies against PRRSV in serum samples. A new method is the detection of PRRSV antibodies in porcine saliva. In this study serum samples and saliva were collected in nine farms suspicious for PRRSV and tested for the presence of PRRSV antibodies. In total 220 serum and 41 saliva samples were taken from pigs at the age of 8 weeks (± 1 week). One saliva and one pooled serum sample (1:5) were tested from each pen. In total 11 (Cut-off 0.4/0.3) or 14 (Cut-off 0.2) serum samples and 23 saliva out of 41 pens were positive for PRRSV antibodies. Cohen`s Kappa testing showed a moderate agreement (κ = 0.446). Saliva samples compared to pooled serum samples were very sensitive, the specificity was 60 and 67, respectively.


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