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Type of publication: Journal Article
Type of document: Full Paper

Year: 2021

Authors: Grünzweil, OM; Palmer, L; Cabal, A; Szostak, MP; Ruppitsch, W; Kornschober, C; Korus, M; Misic, D; Bernreiter-Hofer, T; Korath, ADJ; Feßler, AT; Allerberger, F; Schwarz, S; Spergser, J; Müller, E; Braun, SD; Monecke, S; Ehricht, R; Walzer, C; Smodlaka, H; Loncaric, I

Title: Presence of β-Lactamase-producing Enterobacterales and Salmonella Isolates in Marine Mammals.

Source: Int J Mol Sci. 2021; 22(11):5905



Authors Vetmeduni Vienna:

Bernreiter-Hofer Tanja
Korath Anna
Loncaric Igor
Spergser Joachim
Szostak Michael
Walzer Christian

Vetmed Research Units
Research Institute of Wildlife Ecology, Conservation Medicine
Institute of Microbiology
University Clinic for Swine


Abstract:
Marine mammals have been described as sentinels of the health of marine ecosystems. Therefore, the aim of this study was to investigate (i) the presence of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Enterobacterales, which comprise several bacterial families important to the healthcare sector, as well as (ii) the presence of Salmonella in these coastal animals. The antimicrobial resistance pheno- and genotypes, as well as biocide susceptibility of Enterobacterales isolated from stranded marine mammals, were determined prior to their rehabilitation. All E. coli isolates (n = 27) were screened for virulence genes via DNA-based microarray, and twelve selected E. coli isolates were analyzed by whole-genome sequencing. Seventy-one percent of the Enterobacterales isolates exhibited a multidrug-resistant (MDR) pheno- and genotype. The gene blaCMY (n = 51) was the predominant β-lactamase gene. In addition, blaTEM-1 (n = 38), blaSHV-33 (n = 8), blaCTX-M-15 (n = 7), blaOXA-1 (n = 7), blaSHV-11 (n = 3), and blaDHA-1 (n = 2) were detected. The most prevalent non-β-lactamase genes were sul2 (n = 38), strA (n = 34), strB (n = 34), and tet(A) (n = 34). Escherichia coli isolates belonging to the pandemic sequence types (STs) ST38, ST167, and ST648 were identified. Among Salmonella isolates (n = 18), S. Havana was the most prevalent serotype. The present study revealed a high prevalence of MDR bacteria and the presence of pandemic high-risk clones, both of which are indicators of anthropogenic antimicrobial pollution, in marine mammals.

Keywords Pubmed: Animals
Anti-Bacterial Agentspharmacology
Aquatic Organismsmicrobiology
Bacterial Proteinsgenetics
Bacterial Typing Techniques
Drug Resistance, Bacterial
Enterobacterdrug effectsenzymologygeneticsisolation & purification
Genotype
Mammalsmicrobiology
Microbial Sensitivity Tests
Salmonelladrug effectsenzymologygeneticsisolation & purification
Virulence Factorsgenetics
beta-Lactamasesbiosynthesisgenetics

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